To solve the problem of wrong or inaccurate analyses of severely damaged comet cells by the comet assay image analysis macro software , to implement the function of manual correction to the macro , and to improve the robustness and user friendliness of the software 本研究还增加了对分析错误的数据进行人为校正的功能，由操作者重新指定正确的彗星头中心，从而把错误数据纠正为正确数据，并入统计分析。
And at the beginning of each macro procedure , operation check processes were inserted to minimize the user interference . a standard comet assay was conducted using the improved comet analysis macro , with the intoxication by 100 / / m hydrogen peroxide to mononuclear cells of mouse peripheral blood for 0 , 10 , 20 , 40min respectively to get different dna damage grades 同时，本研究应用改进后软件进行标准彗星试验检测，以100 m过氧化氢染毒小鼠外周血单个核细胞，并经历0 、 10 、 20 、 40min不同时间的孵育，获得不同损伤级别的细胞，并以改进后的软件进行分析。
Dna damages caused by so2 and lead acetate were studied with the single cell microgel electrophoresis technique ( or comet assay ) in order to confirm the damaging degree of lead ( as an important component of atmosphere particle matter ) on dna from male mice exposed to so2 . the migrating distances of dna of brain , lung , spleen and kidney cells of mice increased significantly , compared to the control group under conditions of single and combined poisoning of so2 ( 42mg / m3 ) and lead acetate ( 0 . 2 % ) , and lead could strengthen dna damage degree by so2 in nuclear dna of brain , kidney , spleen cells . damaging degree of so2 on nuclear dna of lung cell of mice was more severe than that of lead 为了明确大气颗粒物中的重要组分? ?铅在二氧化硫所致dna损伤中的作用程度，利用单细胞凝胶电泳技术( singlecellgelelectrophoresis ， scge ，或称彗星实验， cometassay )研究了铅与二氧化硫的联合污染，结果表明在42mg m ~ 3so _ 2和0 . 2醋酸摘要一abstract铅单独及联合染毒条件下，小鼠脑、肺、肾、脾细胞dna迁移距离均比对照显著增加;铅加剧了50 :对脑、肾、脾细胞核dna的损伤程度; 50 :对肺细胞核dna的损伤程度要比铅的损伤大，小鼠肺细胞核dna迁移距离在50 :和醋酸铅联合作用组与醋酸铅单独作用组间有极显著性差异( p < 0 . 01 ) ，而与502单独作用组间没有显著性差异。
